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Research Review: Whey vs Casein

Whey protein digests quickly while casein digests slowly.  In this experiment, researchers tried to determine whether fast digestion or slow digestion is better for protein synthesis and muscle building.  The conclusion?  Fast digestion is best for boosting muscle protein synthesis. Protein powder is a staple for anyone trying to put on muscle. I know that some of you guys looking to gain muscle have giant paint-can-sized tubs of protein powder as furniture in your apartments. (Good for you! Keep eating!)

If you walk into most supplement stores you’ll find more brands, flavors and types of protein than you can imagine.  Of course, most people go with a powder that they can tolerate the taste of, since many brands taste remarkably bad. However, which kind is best?

What is protein powder?

For starters, protein powder is simply processed, dried protein that’s convenient and portable. These powders can be extracted from all sorts of different foods, including:

  • dairy (whey and casein)
  • egg (whole egg or egg white)
  • soy
  • hemp
  • pea
  • even beef
  • etc.

In 2004, dairy-based proteins were the major source of protein in sports supplements — about three-quarters. Soy proteins made up most of the rest.[1]  In recent years, plant based protein powders have become more common.  However, dairy proteins like whey and casein still make up most of the protein supplements on the market.

Whey protein powder — the most common form of dairy-based protein powders — is the liquid by-product of making cheese. Casein, on the other hand, is from the solid part of skim milk when treated with acid (like lemon juice). Casein is what you get when milk curdles — it’s the curd part.

Dairy proteins (as well as egg protein) are considered complete proteins, since they have all essential amino acids in sufficient amounts. A few plant proteins, like hemp, are also complete, but have a lower total amount of protein.

Whey versus casein

Whey and casein have been a staple for bodybuilders for years. Each protein has unique characteristics. But which is better?  Well, just like comparing an economy car and a sports car, deciding which protein is better depends on the purpose of the protein.

In terms of similarities, both are complete proteins and both have identical Protein Digestibility Corrected Amino Acid Scores (PCDAAs; 1.00) [2].  So, to determine which is better, we have to dig deeper.

In terms of differences, whey protein has more leucine, while casein is higher in glutamine (Figure 1). Whey is also absorbed much more quickly than casein.

Figure 1 – Concentrations of isoleucine, leucine, valine, glutamine and arginine in isolated soy protein (ISP), whey protein (WP) and casein. Values are grams per 100 grams of protein. Adapted from (2).

Leucine and glutamine in muscle protein synthesis

Leucine, uniquely, activates skeletal muscle protein synthesis through a series of molecular biology interactions similar to dominos, called the mammalian target of rapamycin (mTOR) pathway. Since leucine is a key amino acid for activating protein synthesis, and since whey has more leucine, it’s a good choice for building muscle mass.

Glutamine is also important in muscle protein synthesis. After exercise, glutamine is depleted in blood plasma and muscle. More muscle glutamine is directly correlated to higher muscle protein synthesis. Glutamine supplementation leads to more growth hormone, which has anabolic affects on muscle.

Absorption rates

The other difference between whey protein and casein protein is how fast the protein is absorbed, and for how long.

Researchers measure blood amino acids to figure out rate of protein absorption. Drinking whey protein causes increases in blood amino acids levels in under an hour, with peak levels at just under 90 minutes. Casein takes longer to increase blood amino acids, but lasts longer, with elevated levels lasting over 300 minutes [3].

Table 1 – Whey vs glutamine: Summary

Whey Casein
Complete protein Yes Yes
High in leucine Yes No
High in glutamine No Yes
Absorption rate Fast Slow
Duration of elevated blood amino acids Short Long

Obviously, comparing whey and casein is complicated. You have different amino acids, different absorption and possibly different effects on the immune system.How can you tell what really matters? One method is to keep all other conditions constant, then change one thing.

In this experiment, researchers used the same protein (constant), but adjusted how long it took for subjects to consume it (change one thing). Or as I like to call it, the chugging versus sipping experiment.

Research question

Today’s research question: Does rate of ingestion — and thus rate of absorption — change the effects of protein supplementation?

West DW, Burd NA, Coffey VG, Baker SK, Burke LM, Hawley JA, Moore DR, Stellingwerff T, Phillips SM.  Rapid aminoacidemia enhances myofibrillar protein synthesis and anabolic intramuscular signaling responses after resistance exercise. Am J Clin Nutr. 2011 Sep;94(3):795-803. Epub 2011 Jul 27.

Methods

Eight moderately active young men drank 25 grams of whey protein following leg extension strength testing (10 rep max) on two occasions.

  • Trial 1: The men chugged the entire 25 grams at once
  • Trial 2: The men drank 2.5 grams 10 times over 200 minutes (every 20 minutes they took a shot of whey), which mimics the slow but long absorption of casein.

Blood amino acid concentration and blood leucine was measured from blood samples. Muscle protein synthesis (fractional synthesis rate; FSR) was measured through a biochemical technique called isotope labeling.

Researchers also measured amounts of specific proteins involved in regulating muscle protein synthesis from muscle biopsies. A biopsy needle the size of the barrel of a pen is used to sample muscle from the outer quadriceps (vastuslateralis).

Results

Either chugging or shooting a total of 25 grams of whey protein both increased essential amino acids and leucine in the blood (Figure 2a & b).

Chugging (bolus) caused a higher peak level of essential amino acids and leucine compared to serial shooting (pulse) but after 5 hours it seems everything evens out. As Figure 2 below shows, chugging and shooting had the same effect on overall levels, based on area under the curve (AUC), which is a way of looking at levels over a period of time rather than just the peak.

Figure 2 – Average blood concentrations of a) essential amino acids and b) leucine after drinking whey protein all at once (BOLUS; 25 grams) or over 200 minutes (PULSE; 2.5 grams X 10 drinks every 20 minutes). From West DW et al. 2011 (Figure 2).

As interesting as blood leucine levels are, we really want to know what happens to protein synthesis in the muscle (aka myofibrillar protein synthesis). Figure 3 below shows the graph of protein synthesis. You can see that the drinking all the whey protein at once (bolus) increased protein synthesis more than spreading out the same amount of whey over 3 hours (pulse).

Figure 3 – Average myofibrillar protein synthesis rate (myofibrillar FSR) after fasting (fasted), BOLUS (1X 25 grams) or PULSE (2.5 grams X 10 drinks every 20 minutes) after exercise. From West DW et al. 2011 (Figure 4).

 

Conclusion

High peak levels of blood leucine and amino acids cause more muscle protein synthesis than blunted but longer elevated levels of blood leucine and amino acids.  So what does that mean for you?

If muscle building is your primary goal, try drinking a fast-digesting protein drink (with at least 25 grams of protein) within 15 minutes of exercising. If you really want to experiment you could drink a second fast-digesting protein drink 2 or 3 hours after the first. It could be that a second drink after blood amino acids are back to normal would cause more muscle protein synthesis by causing a second peak in blood leucine.

[Of course, this study doesn’t directly suggest that casein or any other slow protein has no value.  After all, casein wasn’t even used in this study.  Rather, the researchers tried to mimic casein’s effects by slowing down the absorption of the whey protein.  In fact, slow digestion may be an asset during other times of the day.  However, after exercise, slow proteins might not be best.]

Now, if you can’t digest dairy, opt for lean proteins from whole foods, like eggs, seafood, or lean meats, and keep your post-workout fat intake low and carb intake high to speed absorption. Have some lean protein an hour or two before training as well, so that there are amino acids already starting to float around. If possible, eat your biggest protein meal of the day as soon as possible after heavy training. Even throw in some BCAA (branched chain amino acids) during training.

Bottom line

Drinking 25 grams of fast-digesting whey protein, all at once and immediately after exercise, increases muscle protein synthesis more than drinking 25 grams of whey protein over 3 hours after exercise.

References

  1. NBJ’s Sports Nutrition and Weight Loss Report 2007-2008. Nutrition Business Journal. Boulder CO. New Hope Natural Media, January 2008.
  2. Paul GL. The rationale for consuming protein blends in sports nutrition. J Am Coll Nutr. 2009 Aug;28 Suppl:464S-472S. Review.
  3. Boirie Y, Dangin M, Gachon P, Vasson MP, Maubois JL, Beaufrère B. Slow and fast dietary proteins differently modulate postprandial protein accretion. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14930-5.

 

Protein Powder Read more

All about milk

 In this article we’ll discuss whether milk (and dairy) is an essential food for everyone.  Or whether it’s the dietary evil many make it out to be.
Civilizations began to use milk as a source of nourishment around 8000 BCE.

Although animals used for milk include cattle, goats, sheep, horses, buffalo, yaks, donkeys and camels, cow’s milk is one of the mildest tasting mammalian milks and the most popular.

No culture has ever habitually consumed milk from an animal that didn’t live on grass/leaves, as flesh-eating animals secrete milk with an odd flavor that most people don’t fancy.

Most flesh eating animals also give birth to a litter (think dogs and pigs), which means the mammary system is spread along the length of the torso. Translation: Milking is difficult with big, clumsy human hands.

Cheese is said to have been discovered by an Arab nomad travelling across the desert during the Neolithic period with milk in a container made from an animal’s stomach. The enzymes in the stomach curdled the milk.

Fast forward to the 1800s & 1900s when our relationship with dairy cows changed. Populations increased and the importance of calcium and phosphorus for skeletal health became evident.

Milk was promoted by public education campaigns and doctors as a rich source of these minerals. Doctors considered milk as an “indispensible” component of a child’s diet based on this association.

The industry responded to the demand and milk came from cows crowded into dirty milking sheds. Lots of cows, lots of dirt, and little space meant sick cows.

There was concern of a milk-borne epidemic as this new form of unhygienic milk production took precedence. Dairy farmers tried sterile bottling and disease testing on cows, but problems persisted; thus, pasteurization and refrigeration became common after 1900.
 

Why is milk processing so important?

Milk ferments unless refrigerated. And bacteria and viruses can be transmitted from animals to humans in the course of handling.

Pasteurization

Pasteurization heats milk in a vat to temperatures that microorganisms cannot tolerate.

There are various forms of pasteurization:

  • Original pasteurization (1920s): 145 degrees F for 35 minutes
  • High temperature short time (HTST) pasteurization (1930s): 161 degrees F for 15 seconds
  • Ultra high temperature (UHT) pasteurization (1970s): 280 degrees F for 2 seconds

HTST and UHT are cheap to implement and regulate. With the increasing demand for milk and milk processing, it was no longer cost effective to produce low volume, raw milk. Smaller farms were driven out of business.

Processing milk results in higher amounts of lactose and this is one of the reasons raw milk is promoted by some (although the risk of milk-borne disease increases).

Homogenization

Homogenization crushes milkfat globules so small that they cannot rise to the surface and form a cream layer. This helps mix added fat soluble vitamins, but can turn raw milk rancid, so pasteurization must also take place.

Homogenization didn’t gain acceptance until the 1930s when cardboard and opaque milk containers were introduced. Before then, the cream line was visible through glass bottles and used by consumers to gauge the richness of milk.
 

What you should know about milk production today

Cows have a nine month gestation period and lactate only when they’ve recently given birth, just like humans. In the past, dairy farmers would allow cows a seasonal reproductive cycle, and birth was planned in sync with the new grass of spring.

This way, the mother had lots of nutritious grazing and time to replenish nutrient stores. Grazing is healthier for cows because it provides fresh air and exercise and grass is what the bovine digestive system is built for.

In contrast, industrial production involves feeding cows grain. More grain means more rumen (stomach) acidity, more thirst, diluted milk and ruminal acidosis. Acidosis leads to ulcers, infectious bacteria, inflammation and growth of E. coli. Antibiotics are administered to offset these ailments.

Current dairy producers inseminate cows just a few months after her previous birth, guaranteeing minimal time between pregnancies. When cows produce milk for longer than one year, their immune systems are compromised and milk quality is diminished.

Not only is this uncomfortable for the cow, it increases pregnancy-triggered estrogens in the milk supply. Estrogens can fuel tumor growth.

Scientists find a soup of suspects while probing milk’s link to cancer

Test-tube studies and studies in adults over the past decade have linked cow’s milk with an excess cancer risk in the prostate, and to a lesser extent in the breast and ovaries.

A new study by researchers at the National Cancer Institute assayed grocery-store milk for 15 estrogens: estrone, estradiol and 13 metabolic derivatives of these female sex hormones.

Estrogens can fuel the growth of many tumors, even in the prostate–and estrogen can do this at amazingly tiny concentrations. Identifying how estrogens’ prevalence varies by milk type, and in what chemical form the hormones occur, required a new assay, which the NCI scientists describe in an upcoming issue of the Journal of Chromatography B.

Overall, skim milk had the smallest quantity of free estrogens. However, the conjugated type that dominated skim milk’s profile, 2-hydroxyestrone, is known to be one of the most reactive and potentially risky of the metabolites. That metabolite’s concentration in fat-free milk was second only to buttermilk’s.

There are also other sex hormones in milk—the “male” androgens and insulin-like growth factor 1. Many studies have linked elevated concentrations of IGF-1 with cancer risk.  

A cow’s life

More pregnancy means more calves. Calves are taken within 24 hours of birth at most farms. Since male calves cannot be raised to produce milk, they are used for veal. The veal industry is a by-product of the dairy industry. Female calves replace their mothers and are then sent to slaughter.

The number of dairy cows in the U.S. decreased from 18 million to 9 million between 1960 and 2005. Total milk production increased from 120 billion to 177 billion pounds during the same period. This is due to strategic breeding and pharmaceutical aid.

Life of a cow (1850) Life of a cow (2005)
  • Grazed on pastures
  • Produced 56 pounds of milk/day
  • Milked for 6 weeks after birth
  • 336 pounds of milk per year
  • Milk = $5/gallon
  • Life span of 20 years before dying of natural causes
  • Raised in confined feedlot w/grains
  • Produced 67 pounds of milk/day
  • Milked for 10 months after birth 
  • 20,000 pounds of milk per year
  • Milk = $3/gallon
  • Life span of 3-4 years before being sent to the slaughterhouse. “Spent” dairy cows are used for the cheapest forms of beef.

Milk consumption patterns

Americans are drinking less milk than they used to, as well as more lower-fat milk, but eating more cheese and way more frozen dairy products (aka ice cream).

1909

2001

  • 34 gallons of milk per person (27 gallons whole & 7 gallons lower fat)
  • 23 gallons of milk per person (8 gallons whole & 15 gallons lower fat)
  • 4 pounds of cheese per person
  • 30 pounds of cheese per person
  • 2 pounds of frozen dairy products per person
  • 28 pounds per person

 

What you should know about organic vs. conventional milk

Sales of organic dairy are increasing 20-25% each year. Many people assume that “organic” means better in many respects. In some ways this is true.

Organic cows may be fed better. Although organic cows are supposed to only receive organic feed, farmers are not required to feed the cows grass.

Organic cows are less likely to be given hormones. The use of rBST (growth hormone) is prohibited with organic cows. rBST is sometimes given to “conventional” cattle to promote growth and milk production, but is banned in Europe, Japan and Canada because of concerns over human health and animal welfare.

IGF-1 can increase secondary to rBST, increasing the odds of mastitis and decreasing the life expectancy for cows while promoting cancer in humans.

But organic isn’t synonymous with healthy living conditions or humane treatment of animals.

Organic dairy production in the U.S. is concentrated with only a few producers owned by agribusiness conglomerates. Organic dairy farmers generally use the same breed and feed methods as conventional farmers, including concentrated animal feeding operations. Organic milk is processed the same as conventional.
 

What you should know about milk composition

Cow’s milk is made up of 87% water and 13% solids, including minerals (like calcium and phosphorus), lactose, fats, and proteins (like whey, casein, lactalbumins). Fortification with vitamins A and D is necessary since natural levels are low.

Casomorphins are derived from casein, one of the milk proteins. They have opioid (narcotic) properties (think morphine, oxycodone and endorphins). Casomorphins have addictive properties and decrease bowel motility.

The addictive properties make sense from an evolutionary standpoint as the draw to milk is necessary for infant nutrition, calming and bonding with mom. Human milk casomorphins are about 10 times weaker than those found in cows milk.
 

What you should know about milk and health

Most of us consume our mother’s milk after birth and then transition to cow’s milk. Lactase production diminishes around age 4.

When more than small amounts of un-soured milk enter the GI tract, lactose passes intact to the intestine. This draws water, producing bloating and diarrhea. Some evolutionary biologists believe that those who have the ability to digest lactose were among groups whose ancestors were dairy farmers.

Humans are the only animals who have ever thought of transferring milk from mammary glands of another species to opaque containers and selling it. Using another creature’s milk for food is a peculiar custom indeed and is still not universally accepted. Most interspecies milk substitutions would be disastrous for newborns because of the crucial matches between milk composition and nutrient needs.

While kids believe drinking milk is the key to bone health, scientific reviews acknowledge the following:

“Scant evidence supports nutrition guidelines focused specifically on increasing milk or other dairy product intake for promoting child and adolescent bone mineralization.” (Lanou 2006)

Milk and calcium

In many parts of the world cow’s milk is a negligible part of the diet, and yet, diseases associated with lack of calcium (e.g., osteoporosis, fracture) are uncommon.

In fact, data suggests that calcium rich dairy foods actually increase calcium losses from the body.

How much calcium we get from the diet really isn’t that important, rather, what matters is how much we retain in the body. Populations consuming the most dairy have among the highest rates of osteoporosis and hip fracture in later life.

While cow’s milk can be high in certain nutrients, it’s difficult to argue that it is “essential” for optimal health.

Milk and chronic diseases

Dairy consumption has been associated with cardiovascular diseases, type 1 diabetes, Parkinson’s disease and cancers. Scientists don’t know if this is specifically due to the dairy fat, casein, or the displacement of other nutritious foods.

Nutrition can alter the expression of genes involved in the development of cancer. Casein, a protein found in cows milk, has been linked to different forms of cancer, with strong associations for lymphoma, thyroid cancer, prostate cancer, and ovarian cancer.
 

What you should know about milk and the environment

Dairy cows consume large amounts of food, produce large amounts of waste, and emit methane. In fact, in the San Joaquin Valley in California, cows are regarded as worse polluters than cars.

Still, dairy is slightly more energy-efficient than raising animals for meat.

Conventional farm Organic farm Soy milk
  • 14 calories of fossil fuel energy produces 1 calorie of milk protein
  • 10 calories of fossil fuel energy produces 1 calorie of milk protein
  • 1 calorie of fossil fuel energy produces 1 calorie of organic soybean protein (for soy milk)

 

Summary and recommendations

Is it possible to get milk from humanely treated animals that is nutritious, sustainable and tastes good? Yes.

Is this where most milk comes from? No.

With the amount of dairy consumed in North America, sustainable and humane dairy operations are a near-impossibility. If we can discard the concept that guzzling X ounces of milk a day is an obligation, perhaps we would be free to discover different forms of it, better production methods and ration intake.

The most nutritious and best tasting milk comes from healthy animals that spend most of their time outdoors on fresh pasture eating lots of grass supplemented with hay, root veggies and grains. In theory, organic family farmers might be better stewards of land, water and food. Non-dairy milks are likely just as nutritious and better for the environment.

More milk in the diet doesn’t necessarily improve bone health. Indeed, consuming high amounts of milk from processed sources is associated with various forms of cancer, diabetes, cardiovascular diseases and neurological disorders.
“You have to decide: Is there anything good about milk? Other than developing children and malnourished adults, people probably don’t need milk.”
–Oncologist Michael Pollak, McGill University in Montreal

“The key to a healthful diet is to choose naturally nutrient-rich foods, such as dairy foods, first as part of a balanced diet.”
–National Dairy Council

For extra credit

For mammals (mammals have mammary glands), the first form of nourishment is milk.

Butter is slowly simmered to produce ghee.

Casomorphins from cow’s milk have about 1/10th the pain killing strength of morphine.

Individuals who drink > 2 glasses of milk per day have 3 times the lymphoma risk of those who drink less than 1 glass per day.

Lower fat milks are fortified with vitamin A since the vitamin is fat soluble and disappears with the removal of cream/fat.

In 1929, the milkfat in whole milk ranged from 2.9 percent to 8.4 percent. Whole milk now has an industry standard of 3.25 percent.

Some experts suggest that only certain components of dairy pose health problems, such as the casein. Casein isn’t found in butter or whey.

The word milk comes from the Latin mulgeo, which means to press out by softening with the hand.

Buffalo produce rich milk and more milk than most other animals. The reason it never became a staple for many cultures is due to religious beliefs.

Raw milk contains lower levels of lactose.

Disposable milk containers introduced in the 1930s created more waste.

Milk appealed to nomads due to the ease of transport.

 

Organic dairy update

Between 2000 and 2005, the number of certified organic milk cows on U.S. farms increased by an average of 25%. To meet the demand, organic production is evolving like conventional production.

Large organic dairies with 200 cows or more are a small portion of the organic dairy population, but account for more than 1/3 of organic milk production.

About 2/3 of organic dairies report that 50% of dairy forage comes from pasture.  About 1/3 of organic dairies report that 75% or more of dairy forage comes from pasture.

Using pasture for dairy feed costs less than higher energy feed sources, and average feed costs per cow decline as more pasture is used.

Organic dairies produce about 30% less milk per cow than conventional dairies.  And organic dairies use more pasture-based feeding.

Pasture-based organic dairies’ total economic costs were about $4 per unit higher than conventional pasture-based dairies, much lower than the average price premium for organic milk in 2005.


References

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Huang Z, Himes JH, McGovern PG. Nutrition and subsequent hip fracture risk among a national cohort of white women. Am J Epidemiol 1996;144:124-34.

Cummings SR, Nevitt MC, Browner WS, et al. Risk factors for hip fracture in white women. N Engl J Med 1995;332:767-73.

Finn SC. The skeleton crew: is calcium enough? J Women’s Health 1998;7(1):31-6.

Nordin CBE. Calcium and osteoporosis. Nutrition 1997;3(7/8):664-86.

Reid DM, New SA. Nutritional influences on bone mass. Proceed Nutr Soc 1997;56:977-87.

Tucker KL, Hannan MR, Chen H, Cupples LA, Wilson PWF, Kiel DP. Potassium, magnesium, and fruit and vegetable intakes are associated with greater bone mineral density in elderly men and women. Am J Clin Nutr 1999;69:727-36.

Prince R, Devine A, Dick I, et al. The effects of calcium supplementation (milk powder or tablets) and exercise on bone mineral density in postmenopausal women. J Bone Miner Res 1995;10:1068-75.

Ornish D, Brown SE, Scherwitz LW, Billings JH, Armstrong WT, Ports TA. Can lifestyle changes reverse coronary heart disease? Lancet 1990;336:129-33.

Honglei C, et al. Consumption of dairy products and risk of Parkinson’s disease. Am J Epidem 2007;165:998-1006.

Cramer DW, Harlow BL, Willet WC. Galactose consumption and metabolism in relation to the risk of ovarian cancer. Lancet 1989;2:66-71.

Outwater JL, Nicholson A, Barnard N. Dairy products and breast cancer: the IGF-1, estrogen, and bGH hypothesis. Medical Hypothesis 1997;48:453-61.

Chan JM, Stampfer MJ, Giovannucci E, et al. Plasma insulin-like growth factor-1 and prostate cancer risk: a prospective study. Science 1998;279:563-5.

World Cancer Research Fund. Food, Nutrition, and the Prevention of Cancer: A Global Perspective. American Institute of Cancer Research. Washington, D.C.: 1997.

Cadogan J, Eastell R, Jones N, Barker ME. Milk intake and bone mineral acquisition in adolescent girls: randomised, controlled intervention trial. BMJ 1997;315:1255-69.

Childhood dairy intake and adult cancer risk: 65-y follow-up of the Boyd Orr cohort
van der Pols JC, et al. American Journal of Clinical Nutrition, Vol. 86, No. 6, 1722-1729, December 2007

Gao X, et al. Prospective study of dietary pattern and risk of Parkinson disease.

Scott FW. Cow milk and insulin-dependent diabetes mellitus: is there a relationship? Am J Clin Nutr 1990;51:489-91.

Karjalainen J, Martin JM, Knip M, et al. A bovine albumin peptide as a possible trigger of insulin-dependent diabetes mellitus. N Engl J Med 1992;327:302-7.

Bertron P, Barnard ND, Mills M. Racial bias in federal nutrition policy, part I: the public health implications of variations in lactase persistence. J Natl Med Assoc 1999;91:151-7.

Jacobus CH, Holick MF, Shao Q, et al. Hypervitaminosis D associated with drinking milk. N Engl J Med 1992;326(18):1173-7.

Holick MF. Vitamin D and bone health. J Nutr 1996;126(4suppl):1159S-64S.

Outwater JL, Nicholson A, Barnard N. Dairy products and breast cancer: the IGF-1, estrogen, and bGH hypothesis. Medical Hypothesis 1997;48:453-61.

Mendelson A. Milk. The surprising story of milk through the ages. 2008. Alfred A. Knopf, Random House. New York.

Schmid R. The Untold Story Of Milk. 2009. New Trends Publishing. Washington D.C.

Rhodes D & Morris G. Greener Pastures: Health and Sustainability on a Family Dairy Farm. The HEN Post, Hunger and Environmental Nutrition – a dietetic practice group of the American Dietetic Association. Winter 2009.

Woodford K. Devil in the milk. 2007. Chelsea Green Publishing. White River Junction, VT.

Margen S, et al. Studies in calcium metabolism. I. The calciuretic effect of dietary protein. Am J Clin Nutr 1974;27:584-589.

Kerstetter JE & Allen LH. Dietary protein increases urinary calcium. 1989;120:134-136.

Lanou AJ. Bone health in children. BMJ. 2006;333:763-764.

Leibenluft, J. Is it better for the environment to drink cow’s milk or soy milk?

U.S. Department of Agriculture National Agricultural Statistics Service. Milk Cows: Inventory by Year, U.S.: 1993 to 2002. Accessed January 27, 2006, at www.nass.usda.gov/Charts_and_Maps/Milk_Production_and_Milk_Cows/milkcows.asp.

Raloff J. Scientists find a soup of suspects while probing milk’s link to cancer. Science News. March 28th, 2009.

McBride WD & Greene C.  Characteristics, costs and issues for organic dairy farming.  Economic Research Report No. (ERR-82) November 2009. http://www.ers.usda.gov/Publications/ERR82
 

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